LDL Cholesterol Test

LDL (Low-Density Lipoprotein)

In the world of healthcare, knowledge is power. When it comes to maintaining a healthy lifestyle, being informed about your body's intricacies is essential. One crucial aspect of your health that demands attention is cholesterol, specifically LDL cholesterol.

Low-density lipoprotein (LDL) cholesterol is often referred to as the "bad" cholesterol, and it plays a significant role in your cardiovascular health. Understanding your LDL cholesterol levels can be a critical step toward preventing heart disease and making informed decisions about your well-being. In this comprehensive guide, we will delve into the LDL cholesterol test, shedding light on what it is, why it matters, and how it is conducted. Whether you're a health-conscious individual seeking to optimize your cholesterol levels or someone with a specific medical concern,

 

LDL (Low-Density Lipoprotein) belongs to the lipoprotein particle family. Its size is approximately 22 nm and its mass is about 3 million Daltons.
 
Function:
1. LDL transports cholesterol and triglyceride from the liver to peripheral tissue.
2. LDL also regulates cholesterol synthesis at these sites.
 

Association with disease:

* Atherosclerosis
* Heart attack
* Strock
* Peripheral vascular disease.
LDL TEST:

Sample Collection Procedure:

·       The Vials should be checked for appropriate kinds and for barcode or paper labeling
· Put the patient in a comfortable position with the arm properly supported.
·       Apply a tourniquet above the elbow joint
·       Ask the patients to make a fist S.
·       Think of the four things when feeling for a vein punching bounce, direction of the vein, size of needle, and depth.
·       Clean the area with 70% ethanol and allow dry in air.
·       Check the needle, especially the tip, and check for any blockage by pressing the piston.
·       Grasp the back of the forearm and make the overlying skin tight.
·       The vein is penetrated by positioning the needle at a 15-degree angle.
·       Draw and piston slowly.
·       After blood has been drawn the patients should release the first and the tourniquet is also removed.
·       Press the vein puncture area with the cotton with antiseptic as soon as the needle is removed. The patients may remove the cotton after 7-10 minutes.
·       After removing the needle the collected blood is dispensed in the appropriate tubes.
·       After vein puncture the needle should be removed from the syringe and disposed of by using a needle destroyer. Reagent Preparation: Reagent and standard are ready for use. Test Procedure: Sample 1000 ml Standard
Reagent Preparation:
Reagents & standards are ready for use by company policy.

Analyzer Parameter:

Wavelength / Filter: 578 nm (For Analyzer), 530 nm (For Colorimeter)                            
Method: End Point                                                            
Cuvette : 1cm light path                                                         
Reaction Direction: 10 minutes                                       
Bank: Distilled Water / Reagent                                                                
Reagent Volume (1) : 750   m l
Reagent Volume (2) : 250 m l
Sample Volume (ul): 10   ml
 Normal Low (mg / dl) : 30 mg / dl                                    
Normal High (mg / dl) : 130 mg / dl                                 
Linearity Low (mg/dl): 10 mg/dl                                  
 Linearity High (mg/dl): 1000 mg/dl                              
Units: mg/dl                                                               
Temperature: 37 'c  

LDL Cholesterol

Materials: 

• Cotton
• Hexaso
• Tourniquet
• Gloves
• Marker Pen
• Test tube rack
• Disposable Syringe
• Gel tube / Yellow vial
• Stop Watch
• Calculator
• Colorimeter
• Semi-Auto analyzer
• Incubator
• Micropipette

Test Procedure 
                          Blank           Standard            Sample
Reagent-           750 µl            750 µl             750 µl
Standard            -                     10 µl                     -
Sample              -                       -                       10 µl
DW                  10 µl              -                          -
Mix and well 37'c wait for 5 minutes
Reagent-2          250 ml            250 ml              250 ml
 
The contents of the test tubes are thoroughly mixed and kept at 37 c for 5 minutes wait or room temperature for 10 minutes. the intensity color is measured by a colorimeter or analyzer.
 
Calculation :
LDL of Concentration =  OD of Sample/OD of Standard*Concentration of calibrator(mg/dl)                                                                     
                                                                       
Normal Value :
Up to 130 mg/dl
Suspect = 150 mg/dl
Elevated = 180 mg/dl
 
LDL Calculation:
Total Cholesterol- (HDL Cholesterol + Triglyceride / 5) 
Or
(Triglyceride / 5 + HDL- Total Cholesterol) not to allow results-
\ Caution:
LDL is an important test be care must be taken when testing blood sugar to avoid hemorrhagic blood you have to do QC every day as well as run standard

In conclusion, the LDL cholesterol test is a vital tool in assessing and managing one's cardiovascular health. This test measures the levels of Low-Density Lipoprotein (LDL) cholesterol in the blood, often referred to as "bad" cholesterol. The information obtained from this test is instrumental in understanding an individual's risk of heart disease and making informed decisions about their well-being.

Reagent company name: 

• 1.TRADSWORTH LTD (Human GmbH, Germany)       
• 2. GENETIC TRADING
• 3. MEDI-VISION    LTD  
• 4. BIO-TRADE INTERNATIONAL
• 5. BIO-MEDICAL LIMITED
• 6. MEDITECH
• 7. HEALTH CARE TECHNOLOGIES
• 8. RAJANIGANDHA INTERNATIONAL
• 9. RANDOX
• 10.PLAYMATES
• 11. LEIZ SUPPLY AGENCY
• 12. ABC INTERNATIONAL
• 13. ROSE INTERNATIONAL
• 14. BIOZEN.