Triglycerides Test
Definition: The primary type of fat stored by the body is known as adipose tissue. Adipose tissue is composed of adipocytes, which store energy in the form of triglycerides. A triglyceride comprises three fatty acid molecules bonded to a glycerol molecule. Triglycerides function as the foundation for various lipid types, including fats. These triglycerides originate from the food we consume and are also synthesized within the body.
A triglyceride test is a medical diagnostic test that measures the levels of triglycerides in your blood. Triglycerides are a type of fat (lipid) found in your bloodstream and stored in your body's fat cells. They are one of the main forms of energy storage in the body and are derived from the fats you eat, as well as those produced by the liver.
This test is essential because elevated levels of triglycerides in the blood can be associated with various health risks, particularly cardiovascular disease. Monitoring your triglyceride levels can help assess your risk of heart disease and other health conditions.
Principle of Triglyceride:
Triglyceride- Glycerol + Fatty Acids
Glycerol + ATP-Glycerol-3-Phosphate + ADP
Glycerol-3-Phosphate + O2-dihydroxyacetone phosphate + H2O2
H2O2 + 4-amino antipyrine-Quinoneimine + HCL + H2O + 4-Chlorophenol
Indication of Triglyceride test:
- * Coronary Heart disease
- * Heart attacks
- * Atherosclerosis
- * Angina
- * Obesity
- * Diabetes
- * MI (My cardiac Infraction)
- * Hyperlipidemia/hypercholesterolemia
- * Liver Disease
- * Hypothyroidism
- * Nephrotic syndrome
- * Hypertension
Risk Factors:
- Several factors can contribute to high triglyceride levels, including:
- A diet high in saturated fats, trans fats, and refined sugars
- Obesity or overweight
- Physical inactivity
- Excessive alcohol consumption
- Certain medical conditions like diabetes, thyroid disorders, and kidney disease
- Medications such as steroids, diuretics, and some beta-blockers
Investigation of Triglyceride : Tg, Triglyceride, s. triglyceride, TG,
 |
| Serum Triglyceride |
Sample Collection :
· The Vials should be checked for appropriate kinds and for barcode or paper labeling
· Put the patient in a comfortable position with the arm properly supported.
· Apply a tourniquet above the elbow joint
· Ask the patients to make a fist S.
· Think of four things when feeling for a vein bounce, direction of the vein, size of the needle, and depth.
· Clean the area with 70% ethanol and allow dry in air.
· Check the needle, especially the tip, and check for any blockage by pressing the piston.
· Grasp the back of the forearm and make the overlying skin tight.
· The vein is penetrated by positioning the needle at a 15-degree angle.
· Draw and piston slowly.
· After blood has been drawn the patients should release the first and the tourniquet is also removed.
· Press the vein puncture area with the cotton with antiseptic as soon as the needle is removed. The patients may remove the cotton after 7-10 minutes.
· After removing the needle the collected blood is dispensed in the appropriate tubes.
· After vein puncture the needle should be removed from the syringe and disposed of by using a needle destroyer. Reagent Preparation: Reagent and standard are ready for use. Test Procedure: Sample 1000 ml Standard
Methods of S. triglyceride Estimation: Endpoint
Reagent Preparation: All reagents are ready for use.
Stability of working reagent: From 2-8 degrees Celsius temperature express date.
Reagents required: 1. Triglyceride reagent2.Standard
Concentration Of standard: 200 mg/dl
Materials:
- Cotton
- Hexaso
- Tourniquet
- Gloves
- Marker Pen
- Test tube rack
- Disposable Syringe
- Gel tube / Yellow vial
- Stop Watch
- Calculator
- Colorimeter
- Semi-Auto analyzer
- Incubator
- Micropipette
Analyzer Parameter:
Wavelength / Filter: 530-546 nm
Method: End Point
Cuvette : 1cm light path
Reaction Direction: 30 minutes
Bank: Reagent
Reagent Volume: 1000 microliters
Sample Volume (ul): 10 microliters
Normal Low (mg / dl) : 40
Normal High (mg / dl) : 150
Linearity Low (mg/dl): 10
Linearity High (mg/dl): 1000
Units: mg/dl
Test Procedure:
Blank Standard Sample
Reagent triglyceride 1 ml 1ml 1ml
Blank (Reagent) 10 microliter - -
Standard - 10 microliter -
Sample - - 10 microliter
Mix well in 10 minutes at room temperature or 5 minutes at 37 * c incubator then read the absorbance (OD) of the sample and standard against the reagent blank
Calculation and curve:
Concentration of Triglyceride = OD of Test / OD of standard * concentration of standard (200 mg/dl)
Normal Value: Up to 165 mg/dl
Interpretation:
Triglyceride levels are typically measured in milligrams per deciliter (mg/dL) of blood. The interpretation of the results can vary depending on various factors, including age, sex, and overall health. However, in general:
Normal: Less than 150 mg/dL
Borderline high: 150 to 199 mg/dL
High: 200 to 499 mg/dL
Very high: 500 mg/dL or higher
Hypertriglyceridemia: in which there is a high level of triglyceride is called hypertriglyceridemia.
In conclusion, the triglyceride test is a valuable tool for assessing cardiovascular health and identifying potential risks. Monitoring and managing triglyceride levels, along with other lipid profile components like cholesterol, can help individuals take proactive steps to reduce their risk of heart disease and improve overall well-being. It's essential to discuss your test results and any recommended interventions with your healthcare provider.
Caution:
Triglyceride is an important test care must be taken when testing blood sugar to avoid hemorrhagic blood you have to do QC every day as well as run standard
1 .TRADSWORTH LTD (Human GmbH, Germany)
2. GENETIC TRADING
3. MEDI-VISION LTD
4. BIO-TRADE INTERNATIONAL
5. BIO-MEDICAL LIMITED
6. MEDITECH
7. HEALTH CARE TECHNOLOGIES
8. RAJANIGANDHA INTERNATIONAL
9. RANDOX
10.PLASMATEX
11. LEIZ SUPPLY AGENCY
12. ABC INTERNATIONAL
13. ROSE INTERNATIONAL
14. BIOZEN.
