Blood collection
Blood collection procedures can vary depending on the purpose of the collection and the type of sample needed (e.g., whole blood, serum, plasma). Here is a general procedure for various types of blood collection, but it's essential to follow specific guidelines and recommendations from your local health authority or healthcare organization as they may have slight variations:
Definition: Removal of free flow of blood, usually by venipuncture laboratory technologist or phlebotomy, venous blood sampling. common term for blood sampling collection for laboratory analysis is called blood collection.
According to the site: There are three sites
1. Capillary Blood Collection
2. Venous Blood Collection
3. Artial Blood Collection
Prepare the Patient:
1. Position the patient comfortably, with their arm extended and palm up (for venipuncture). 2. Ask the patient to clench and release their fist several times (for venipuncture) to help locate a suitable vein.
Venous Blood Collection
Materials:
* Cotton
* Hexasol or 70% Alcohol,
Blood collection procedure of Venous:
· The Vials should be checked for appropriate kinds and for barcode labeling paper-d'Or
· Put the patient in a comfortable position with the arm properly supported.
· Apply a tourniquet above the elbow joint
· Ask the patients to make a first
· Think of four things when feeling for a vein bounce, direction of the vein, size of the needle, and depth.
· They are cleaned with an area of 70%: ethanol and are allowed to dry in the air.
· Check the needle, especially the tip, and check for any blockage by pressing the piston.
· Grasp the back of the forearm and make the overlying skin tight.
· The vein is penetrated by positioning the needle 15, a degree angle to have Red Hat.
· Draw and piston slowly.
· After blood has been drawn the patients should release the first and the tourniquet is also removed.
· Press the vein puncture area with the cotton with antiseptic as soon as the needle is removed. The patients may remove the cotton after 7-10 minutes.
· After removing the needle the collected blood is dispensed in the appropriate tubes.
· After vein puncture the needle should be removed from the syringe and disposed of by using a needle destroyer.
Reagent Preparation: Reagent, standard, and quality control are ready for use.
Test Procedure: Sample 1000 ml Standard
Blood collection procedure Of Capillary:
Material:
1. Sterile blood collection tubes (varies by test) 2. Sterile needles (varies by collection method) 3. Syringes (if necessary) 4. Alcohol swabs 5. Gauze or cotton balls 6. Tourniquet (optional) 7. Bandages or adhesive tape 8. Biohazard waste container 9. Gloves 10. Patient identification labels 11. Request forms (with patient information)
1. Identify the patient, Two forms of active identification are required. ask for the patient's name, location, and date of birth. This information must match the requisition.
2. Reassure the patients that the minimum or scanty amount of blood required for testing will be drawn.
3. Select the appropriate blood lancet for the specimens to be collected. Any disposable blood lancet containing additives should be tapped to dislodge additives from the walls and tap.
4. Wash hands and put on hand gloves.
5. Position the patient with the arm extended to form a straight line from shoulder to wrist during the stay.
6. Do not try to attempt a capillary vein puncture more than twice.
Heel puncture:
1. Position the infant or child with the head slightly elevated
2. Normal Condition the heel from which blood is to be obtained. Many times a commercial heel warmer is done.
3. Cleanse the heel with 70% alcohol prep, then dry.
4. Using a sterile blood lancet, puncture the most medial or lateral portion of the plantar surface of the mid-great toe to the heel.
5. Puncture no deeply than 2.4mm (approximately 0.1 inches).
7. Previous puncture sites should be avoided
8. Clean away the first drop of blood with sterile gauze.
9. When finished, When finished, clean the puncture site and apply pressure with clean gauze to stop the bleeding. Apply an adhesive bandage.
12. Label all specimens per the accepted registration ID.
13. Place labeled specimens in a zip-lock bag and deliver them to the laboratory as soon as possible.
1. Position the patient suitably so that the hand is easily accessible.
2. Cleanse the fingertip of the 3rd (middle finger) or 4th (ring finger) with a 70% alcohol pad. Allow the finger to dry a sterile gauze.
3. Using a sterile blood lancet, puncture the fingertip in the fleshy part of the finger, slightly to the side of the center and across (perpendicular to) the fingertip. The blood forms as a drop on the fingertip. If the puncture is parallel to the lines of the fingerprint, the blood will not form as a minimum drop but will run down the finger making collection difficult.
4. Clean away the first drop of blood with a sterile gauze.
5. Allow another maximum drop of blood to form. Lightly touch the
filter paper to the large drop of blood. Collect drops of blood into the collection device by gently massaging the finger and then sterile cotton for the cleaning. Avoid excessive pressure from which tissue fluid comes. into the drop of blood.
6. When finished, clean the sterile cotton and apply pressure with clean gauze to stop the bleeding. apply an adhesive bandage.
7. Label all specimens per accepted guidelines.
8. Place labeled specimens in zip-lock bags and deliver them to the laboratory as soon as possible
Filter paper sample collection:
1. Allow the blood to soak through and completely fill the pre-printed circle on the filter paper waiting a few minutes.
2. Filter paper should touch only the blood and not the skin touching in filter paper.
3. Apply only one drop of blood per circle on filter paper. Do not add blood to a circle filled with blood.
4. Apply must be blood to the printed side of the filter paper.
5. Make certain that the blood completely saturates all four circles and is visible from both
sides.
6. If the blood flow is diminished, repeat the capillary Puncture to the collection.
7. Allow filter paper to air dry for two hours at room temperature. Avoid placing samples on
hot surfaces such as monitors.
8. Forward completed collections to the laboratory as soon as possible.
1. Dispose of all sharps in a designated biohazard waste container. 2. Remove gloves and dispose of them properly. 3. Ensure that all collected samples are correctly labeled.
Documentation: 1. Record the date, time, and location of the blood collection. 2. Complete the necessary paperwork and request forms.
SAFETY
1. Observe standard safety precautions. Observe all applicable isolation
procedures in the supervision.
2. PPE will be caught at all times.
3. Wash hands in caught, tap water with an appropriate hand washing product, if not visibly contaminated, with a commercial foaming hand wash product distributor instruction before and after each patient collection in the laboratory.
4. Gloves are to be caught during all blood collection procedures and changed between
patient collections.
5. A laboratory coat must be caught during blood collection procedures.
6. Lancets are disposed of in an appropriate container as one unit.
7. Gloves are to be discarded in the appropriate container quickly after the blood
collection procedure.
8. All laboratory materials and items used for the procedure must be disposed of according to the proper biohazard waste disposal policy learned by medical technologists.
9. Contaminated surfaces must be cleaned with freshly prepared viodine solution. All surfaces are cleaned daily with antiseptic.
10. In the case of an emergency stick, quickly wash the area with a bar of antibacterial soap, express blood from the wound, and contact your medical technologist.
